ANTISICKLING ACTIVITY EVALUATION OF FRACTIONS OBTAINED FROM WHOLE EXTRACTS OF NEWBOULDIA LAEVIS P. BEAUV (BIGNONIACEAE )
By: Dermane, Affo
.
Contributor(s): Kepegba, Kafui.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(2).Description: 42-46p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
This study aims to
evaluate the fractions from whole extracts of roots and stem barks of
Newbouldia
laevis
and their effect on sickling
.
Method
s:
Hydroethanolic
extracts
of stem bar
ks and root
s of
Newbouldia
laevis
we
re fractionated by
the technics of cold precipitation in ethanol.
The fractions obtained after phytochemical
screening were subjected to
Emmel test to evaluate their anti
-sickling activity. Active fractions were
tested for DPPH and AAPH assay
(AAPH induced m
embrane
lipoperox
idation
and evaluation of
reduction of
hemolysis)
.
Result
s:
Two fractions were obtained from each whole extrac
t: supernatant and pellet fractions.
Supernatants fractions obtained from whole roots
bark
s extract and stem barks extract
at a concentration of 3
0 mg
/ml
reduced sickling up respectively to 7
% and
10%
against 86% for the control
.
Pellets fractions obtained from
the both
extracts induced coagulation of SS blood
at 30 mg
/ml
against 86% for the control
.
Conclusio
n:
Supernatants fractions of hydroethano
lic whole extract of roots and stem backs of
Newbouldia
laevis
promise as the potential source of
active molecules against sickle cell disease.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020886 |
Objective:
This study aims to
evaluate the fractions from whole extracts of roots and stem barks of
Newbouldia
laevis
and their effect on sickling
.
Method
s:
Hydroethanolic
extracts
of stem bar
ks and root
s of
Newbouldia
laevis
we
re fractionated by
the technics of cold precipitation in ethanol.
The fractions obtained after phytochemical
screening were subjected to
Emmel test to evaluate their anti
-sickling activity. Active fractions were
tested for DPPH and AAPH assay
(AAPH induced m
embrane
lipoperox
idation
and evaluation of
reduction of
hemolysis)
.
Result
s:
Two fractions were obtained from each whole extrac
t: supernatant and pellet fractions.
Supernatants fractions obtained from whole roots
bark
s extract and stem barks extract
at a concentration of 3
0 mg
/ml
reduced sickling up respectively to 7
% and
10%
against 86% for the control
.
Pellets fractions obtained from
the both
extracts induced coagulation of SS blood
at 30 mg
/ml
against 86% for the control
.
Conclusio
n:
Supernatants fractions of hydroethano
lic whole extract of roots and stem backs of
Newbouldia
laevis
promise as the potential source of
active molecules against sickle cell disease.
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